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Orienting Crystals with the 1-CCD Laue Camera

To control this hi-res CCD, use either the native Artemis Capture application (which can be downloaded with the Atik core software), the ImageJ imaging environment, the LabView graphical instrument control environment, or the Artemis SDK using MS Visual Basic, C++.

  • Sensor Type: Sony ICX825ALA or ICX694ALG
  • Image size: Diagonal 11mm (Type 2/3") or 16mm (type 1")
  • Chip size: 8.98x6.71mm or 12.49x9.99mm
  • Resolution: 1392x1040 or 2759 x 2200
  • Pixel Size: 6.45x6.45 µM or 4.54 x 4.54 µM
  • Binning: from 2x2 to 8x8 (improved intensity and read-out )
  • High sensitivity: (QE>75% at 500-600nm), low smear
  • Low dark current: 0.003@-10 °C, excellent anti-blooming
  • Full well capacity: 20,000 or 40,000 electrons
  • ADC: 16 bit grey scale image, optional filtering and distortion
  • Readout Noise: 4 e- typical (slower readout = less noise)
  • Readout Time: 1-3s
  • Interface: USB 2.0 High Speed with 10-20m USB cables
  • Power: 12v DC 0.8A, to EU, UK, US/Japan, AU/CN standards
  • Maximum Exposure Length: Unlimited
  • Minimum Exposure Length: 1/1000s (1s at full resolution)
  • Cooling: Thermoelectric set point with max ΔT=-27°C
  • CCD Unit: 60mm diameter, 122mm length, 400g weight
  • Lens Coupling: T2-mount, C-mount, F-mount, micro 4/3
  • SDK: C++, VB Wrapper, .net Wrapper, ImageJ, LabView

Setting up the 1-CCD Laue Camera

Mount the camera as close to the source as possible using the 4mm bolt holes on the back; minor adjustments to position and orientation should be provided. Before inserting the 1mm collimator tube into the camera, check that it fits the source exit and allows no x-ray leakage. The carbon fibre window and plastic scintillator are not fragile.

The camera and collimator must be precisely aligned, and the source voltage and current set for maximum x-ray intensity through the collimator.

Our mini i-Cam camera could be used to maximise the beam and position the sample, otherwise use the Laue camera itself to image transmission through the collimator.
With this single CCD camera, just follow the instructions for the high resolution CCD and then skip to Indexing

Typical exposure times for backscattered Laue are ~300 seconds. But first try a shorter exposure with no beam to check that everything is working - you should see background noise. You can also try forward scattered Laue, where exposure times are much shorter.

Binning is important for faster exposures with backscattered Laue, since very high resolution is not needed . Typically binning of 4x4 can be used to increase efficiency by a factor of x16 ! Try also the Infinity software, which allows you to see the image as it is built up by stacking shorter exposures.
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If you have problems with collecting images...

  • Try a 1s exposure to check that it works before starting a long exposure
  • Use a Windows-7 or -10 computer with at least 2GByte of memory
  • Close other aplications and windows that may use memory
  • Try unplugging and re-plugging the USB cable
  • Try re-booting your computer :-)
  • Check the files that are created in your image directory
  • Note that the lens distortion correction is not perfect far from the center

Indexing and orienting the Laue pattern

You may first want to use ImageJ's "Process/Noise/Despeckle" to filter out electronic noise and perhaps "Process/FFT/Bandpass filter" to emphasise the peaks, before using imageJ to convert to the various formats used for indexing. A number of Laue indexing applications are freely available. The Cologne Laue Indexing Program (CLIP) is one of the best, along with the QLaue Indexing Program. Older applications such as OrientExpress and the ambitious new ESMERALDA Laue suite might also be tried.